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KMID : 0357319930280010023
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Journal of the Korean Society for Microbiology
1993 Volume.28 No. 1 p.23 ~ p.36
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Cloning and DNA Sequencing of Flagellin Gene of Helicobacter pylori
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이광호
이우곤/조명제/도영미/백승철/강경희/박필성/이상룡
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Abstract
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A λgtll expression library of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen
expressed
from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypeptide with a predicted molecular size of 54kd. This
open
reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homology searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other
flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.
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KEYWORD
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